Comparison and Recommendations for Use of Dientamoeba fragilis Real-Time PCR Assays
نویسندگان
چکیده
منابع مشابه
Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.
A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays posi...
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Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5' nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic exami...
متن کاملReal-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
متن کاملStudy of Dientamoeba Fragilis by Iron Hematoxylin Staining and Nested-PCR Methods in Chalous Healthcare Refers
Abstract Bachground and objectives: Dientamoeba fragilis is a habitant protozoa in human colon causing clinical symptoms, such as local stomach pain, weight loss, lack of appetite and flatulence. It is important to diagnose this infection correctly and differentiate it from other Protozoa. In this study PCR and Iron Hematoxylin methods were used to detection of this protozoa in Chalous Medical ...
متن کاملAdvances on Dientamoeba fragilis Infections
Dientamoeba fragilis is an enteric protozoan parasite that remains neglected, probably due to the misconception that it is uncommon and non-pathogenic. As more information be‐ came available and antimicrobial agents were developed with activity against this para‐ site, it became clear that D. fragilis is responsible of an active infection, associated with symptoms such as abdominal pain and dia...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2019
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01466-18